Journal: Dose-Response

Article Title: Cardamonin Promotes the Apoptosis and Chemotherapy Sensitivity to Gemcitabine of Pancreatic Cancer Through Modulating the FOXO3a-FOXM1 Axis

doi: 10.1177/15593258211042163

Figure Lengend Snippet: The effect of CAR on FOXO3a-FOXM1. PANC-1 and SW1990 cell lines were treated with CAR (0 to 20 μM) for 48 hours. (A) WB was carried out to gauge the PI3K/AKT/mTOR expression. (B) The profile of the FOXO3a/FOXM1 pathway was checked by WB. (C) The interaction between FOXO3a and FOXM1 was confirmed by IP * P < .05, ** P < .01, *** P < .001 (vs Control group), N = 3. CAR: cardamonin, WB: western blot.

Article Snippet: After incubation with the anti-FOXM1 antibody (BD Pharmingen) at 4°C, the cells were further incubated with the protein G Plus/Protein A agarose suspension (Merck) at 4°C for 4 hours.

Techniques: Expressing, Western Blot

Journal: Dose-Response

Article Title: Cardamonin Promotes the Apoptosis and Chemotherapy Sensitivity to Gemcitabine of Pancreatic Cancer Through Modulating the FOXO3a-FOXM1 Axis

doi: 10.1177/15593258211042163

Figure Lengend Snippet: Knocking down FOXO3a reduced GC cells’ chemosensitivity to GEM. (A-B) The FOXO3a knockdown cell model was constructed in PANC-1 and SW1990 cells. (A) WB was performed to monitor the expression of the FOXO3a/FOXM1 pathway after FOXO3a knockdown. (B-C) CCK8 assay was applied to examine PC cells’ viability. (D-E) PC cells with lower levels of FOXO3a were treated with 0-4 μg/mL GEM, for 24 hours. PC cells’ viability was measured by CCK8 assay. P > .05, * P < .05, ** P < .01, *** P < .001 (vs si-NC group), N = 3. WB: western blot, GEM: gemcitabine.

Article Snippet: After incubation with the anti-FOXM1 antibody (BD Pharmingen) at 4°C, the cells were further incubated with the protein G Plus/Protein A agarose suspension (Merck) at 4°C for 4 hours.

Techniques: Construct, Expressing, CCK-8 Assay, Western Blot

Journal: Dose-Response

Article Title: Cardamonin Promotes the Apoptosis and Chemotherapy Sensitivity to Gemcitabine of Pancreatic Cancer Through Modulating the FOXO3a-FOXM1 Axis

doi: 10.1177/15593258211042163

Figure Lengend Snippet: Down-regulating FOXO3a reversed CAR-mediated anti-tumor effects in PC cells. PANC-1 cells transfected with si-NC or si-FOXO3a were treated with 20 μM CAR for 48 hours respectively. (A) CCK8 assay was adopted to measure cell viability. (B) The colony formation experiment was conducted to test the colony-forming ability of PANC-1 cells. (C) PC cells with lower levels of FOXO3a were treated with 20 μM CAR and 0-4 μg/mL GEM. Cell viability was testified by CCK8 assay. (D) WB was conducted to monitor FOXO3a-FOXM1 profiles in PANC-1 cells. *** P < .001 (vs control group); ## P < .01, ### P < .001 (vsCAR+si-NC group). CAR: cardamonin, GEM: gemcitabine, WB: western blot, CCK8: cell counting kit-8.

Article Snippet: After incubation with the anti-FOXM1 antibody (BD Pharmingen) at 4°C, the cells were further incubated with the protein G Plus/Protein A agarose suspension (Merck) at 4°C for 4 hours.

Techniques: Transfection, CCK-8 Assay, Western Blot, Cell Counting

Journal: Dose-Response

Article Title: Cardamonin Promotes the Apoptosis and Chemotherapy Sensitivity to Gemcitabine of Pancreatic Cancer Through Modulating the FOXO3a-FOXM1 Axis

doi: 10.1177/15593258211042163

Figure Lengend Snippet: CAR curbed PC cells’ growth in vivo . PANC-1 and SW1990 cells were subjected to xenograft tumor experiment in nude mice, which were then treated with CAR (5 μg/kg body weight) or the same volume of saline by intraperitoneal injection. (A-F) The tumor images formed in the nude mice. The weight and volume of the tumors were calculated. (G-H) WB was conducted to measure the FOXO3a-FOXM1 and PI3K/AKT/mTOR pathways in the formed tumor tissues. I. Immunohistochemistry was used for detecting FOXO3a-FOXM1 in the tumor tissues. ** P < .01, *** P < .001. N = 5. CAR: cardamonin, WB: western blot.

Article Snippet: After incubation with the anti-FOXM1 antibody (BD Pharmingen) at 4°C, the cells were further incubated with the protein G Plus/Protein A agarose suspension (Merck) at 4°C for 4 hours.

Techniques: In Vivo, Injection, Immunohistochemistry, Western Blot

Journal: Dose-Response

Article Title: Cardamonin Promotes the Apoptosis and Chemotherapy Sensitivity to Gemcitabine of Pancreatic Cancer Through Modulating the FOXO3a-FOXM1 Axis

doi: 10.1177/15593258211042163

Figure Lengend Snippet: The Specific Primer Sequence.

Article Snippet: After incubation with the anti-FOXM1 antibody (BD Pharmingen) at 4°C, the cells were further incubated with the protein G Plus/Protein A agarose suspension (Merck) at 4°C for 4 hours.

Techniques: Sequencing

Journal: Journal of Cancer

Article Title: Lasalocid inhibits melanoma by down-regulating FOXM1 through PI3K/AKT and JNK/P38 MAPK pathways

doi: 10.7150/jca.101798

Figure Lengend Snippet: FOXM1 is a key gene in the inhibition of melanoma by lasalocid (A) Volcano plot of differential genes. (B) KEGG pathway analysis of differentially expressed genes. (C) GO functional enrichment analysis of differentially expressed genes. (D) Venn diagram was used to screen the key genes of lasalocid inhibiting melanoma. (E) GEPIA2 online analysis platform was used to verify the candidate genes.

Article Snippet: Afterwards, the melanoma cells were blocked with BSA blocking solution (Solarbio, China) for 30 min and then incubated overnight at 4°C with anti-FOXM1 antibody (Sanying, China).

Techniques: Inhibition, Functional Assay

Journal: Journal of Cancer

Article Title: Lasalocid inhibits melanoma by down-regulating FOXM1 through PI3K/AKT and JNK/P38 MAPK pathways

doi: 10.7150/jca.101798

Figure Lengend Snippet: Lasalocid inhibits the proliferation, migration and invasion of melanoma cells by down-regulating FOXM1. (A,B) The inhibition of FOXM1 mRNA and protein expression by lasalocid in human melanoma cells was assessed using real-time PCR and Western blot techniques. (C,D) Immunofluorescence assay was used to detect the inhibition of FOXM1 protein expression by lasalocid. (E,F) The mRNA expression of FOXM1-regulated genes in human melanoma cells treated with lasalocid for 24 h was quantitatively detected using real-time quantitative PCR. (G,H) Western blot was used to detect the expression of FOXM1-regulated proteins in human melanoma cells treated with lasalocid for 24 h. (Data are mean±SD * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001).

Article Snippet: Afterwards, the melanoma cells were blocked with BSA blocking solution (Solarbio, China) for 30 min and then incubated overnight at 4°C with anti-FOXM1 antibody (Sanying, China).

Techniques: Migration, Inhibition, Expressing, Real-time Polymerase Chain Reaction, Western Blot, Immunofluorescence

Journal: Journal of Cancer

Article Title: Lasalocid inhibits melanoma by down-regulating FOXM1 through PI3K/AKT and JNK/P38 MAPK pathways

doi: 10.7150/jca.101798

Figure Lengend Snippet: FOXM1 was down-regulated by lasalocid through inhibition of PI3K/AKT pathway. (A,B) The protein expression levels of PI3K, AKT, P-PI3K, and P-AKT in melanoma cells were assessed after 24 h of lasalocid treatment using Western blot analysis. (C,D) Western blot analysis was employed to assess the expression levels of PI3K, AKT, P-PI3K, P-AKT and FOXM1 following activation of the PI3K/AKT pathway by IGF1 (100 ng/mL, 24 h). (E,F) Statistical diagram of the proliferation ability of melanoma cells significantly increased by IGF1 detected by CCK-8. (Data are mean±SD * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001).

Article Snippet: Afterwards, the melanoma cells were blocked with BSA blocking solution (Solarbio, China) for 30 min and then incubated overnight at 4°C with anti-FOXM1 antibody (Sanying, China).

Techniques: Inhibition, Expressing, Western Blot, Activation Assay, CCK-8 Assay

Journal: Journal of Cancer

Article Title: Lasalocid inhibits melanoma by down-regulating FOXM1 through PI3K/AKT and JNK/P38 MAPK pathways

doi: 10.7150/jca.101798

Figure Lengend Snippet: Lasalocid inhibits the proliferation of melanoma cells by activating JNK/P38 MAPK pathway and down-regulating FOXM1. (A,B) The protein expression levels of JNK, P-JNK, P38 and P-P38 in melanoma cells treated with lasalocid for 24 h were detected by Western blot. (C,D) The expression of P38, P-P38 and FOXM1 proteins and the proliferation ability of melanoma cells after inhibiting the P38 MAPK pathway by SB202190 (20 μM, 24 h). (E,F) The expression of JNK, P-JNK and FOXM1 proteins and the proliferation ability of melanoma cells after inhibiting JNK MAPK pathway by SP600125 (20 μM, 24 h). (Data are mean±SD * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001.

Article Snippet: Afterwards, the melanoma cells were blocked with BSA blocking solution (Solarbio, China) for 30 min and then incubated overnight at 4°C with anti-FOXM1 antibody (Sanying, China).

Techniques: Expressing, Western Blot

Journal: Journal of Cancer

Article Title: Lasalocid inhibits melanoma by down-regulating FOXM1 through PI3K/AKT and JNK/P38 MAPK pathways

doi: 10.7150/jca.101798

Figure Lengend Snippet: Lasalocid inhibits melanoma cell proliferation in vivo (A) Schematic representation of in vivo experiments. (B) Photos of nude mice after tumor formation. (C) Images of the stripped tumor. (D) Statistical plot of tumor mass. (E) Statistical plot of mouse weight. (F) Statistical plot of mouse weight. (G) Immunohistochemistry was used to detect the expression of FOXM1, MPP2, MMP9 and Ki67 protein in the tumors. TUNEL staining was used to detect the apoptosis of tumors in vivo . (H) Heart, liver, spleen, lung and kidney tissues were examined by HE to assess the systemic toxicity of lasalocid. (Data are mean±SD * P <0.05, ** P <0.01).

Article Snippet: Afterwards, the melanoma cells were blocked with BSA blocking solution (Solarbio, China) for 30 min and then incubated overnight at 4°C with anti-FOXM1 antibody (Sanying, China).

Techniques: In Vivo, Immunohistochemistry, Expressing, TUNEL Assay, Staining